0 OD for earlier models. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. An ampicillin-resistant E. The SpectraMax 190 reader can be integrated with Molecular . Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. Learn more about their features and specifications in. Search. #4TI-0214) UltraVision Plate 96 (Brooks Life. Click on each for more information. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. 9. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. At the beginning of a data run, perform a ìsignal testî to check the basal fluorescence signal (i. The Synergy H4 is a convenient benchtop microplate reader that offers robust. One plate carrier adapter is provided with the instrument. The automated ELISA workflow has two positive outcomes. NORTH AMERICA. We would like to show you a description here but the site won’t allow us. #Flex3) Methods. It features a modular design that allows users to add or change detection cartridges according to their needs. Open the top cover of the instrument. (a) Chemical structures of 1 (HADP) and its analogues 2–4. begin reading microplates with seven mouse clicks. ; SoftMax® Pro 7. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Test up to 3. SoftMax Pro 7. Step 2. 1. 9. Select the Driver tab. Supplier: Molecular Devices The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes. Measure more accurately. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. To update the driver. 635. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. SAN JOSE, Calif. Find regional support. and California)Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Combining multiple microplate technologies and detection modes into a single, more versatile unit may be an ideal choice especially if you have. Molecular Devices training courses and tutorials. Temperature control and shaking come standard. Page 1 of 8 . The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. When connecting for the first time to either the SpectraMax L reader or StakMax system. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Molecular Devices' microplate readers. Versa Max. 25 to 3. 58% for the detection module and 2. Turn the reader on and secure the USB cable. Avoid losing high signal. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. The SpectraMax ABS Plus reader utilizes Molecular . The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. They feature options and modules to address your specific research including objectives, filters, imaging modes, and environmental. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human. The Gemini instruments can read some luminescence but it is about 100 times less sensitive than a dedicated luminometer. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Exploring misconceptions and understanding the technologies. First, as mentioned before, the walkaway time significantly. Shaking. 1 sec. Plate reader. 5: Schematic of the back panel of a reader. Endpoint, kinetic, spectrum. Absorbance Plate Reader Comparison Chart. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Plate reader settings used for data acquisition of bacterial growth curve. PURPOSE. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. ©2012 Molecular Devices, LLC | 8/12 | Printed in USA | PN: 0120-1527. HTRF-compatible SpectraMax Multi-Mode Readers. 2, 2022 – Molecular Devices announced today the launch of the ValitaCell IgG titer assay, ValitaTiter, available for global distribution. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Complete plate reader solution for all your research needs. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. 425 or. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. S. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. FlexIII. Eight filter options provide simplified assay setup. Supplier: Molecular Devices. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. We would like to show you a description here but the site won’t allow us. Products : General, FilterMax F5 reader. 40266 Email: drugdiscoverytech@lifetech. 2 only) SpectraMax M-series Microplate readers. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Also refer to the Multimode Reader Comparison Chart Features. Read height should be optimized forIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Contact Us Request Quote Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. 1. The calculated LOQ values were 0. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Laboratory automation workflows can help. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Supersedes 1. B. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. The plate reader optics are. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Turn on Plate Reader and wait for it to complete its start-up routine. Timothy Bolus, MPM, MBA, Compliance Program Manager. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. 0 × 103 to 2. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. For biochemical assays, the washer removes unbound material and unreacted reagents. Fig 2: Enzymatic response of synthetic compounds. 9. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. We therefore gathered salient. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical. 5577 UK +44. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Instrument settings can be saved as a protocol file. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). BMG Labtech - THERMOstar. Installation Qualification (IQ)the iD5 reader’s filter system. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Weight. SoftMax Pro GxP Software. you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Toggle navigation 0 Cart; Store. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Recertification service. Explore our fully-integrated,. 400×320×350 mm. #Flex3) FlexStation 3 8-channel Pipettor Head (Molecular Devices cat. For more sample throughput, the system can be easily integrated into a full roboticThe SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 211, and 820 and EudraLex Volume 4 and Annex 11 and 15, and how they apply to the implementation of Molecular Devices microplate readers and SoftMax Pro. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Three low volume 24-well bottom slides. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. . To update the driver. 6% for the on-board optics (monochromators). Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. That’s why Molecular Devices offers the following services: Installation qualification (IQ), operational qualification (OQ), preventive maintenance (PM), and repair coverage. The Back Panel Figure 1. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Uncontrolled Copy for Training and Reference Purposes Only . (a) Chemical structures of 1 (HADP) and its analogues 2–4. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Download PDF. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. FLIPR. 5 hours. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Figure 2. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). EUROPE. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Sale price $18,499. The adapter is required for optimum performance with standard 96-well and 384-. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. User selects appropriate wells for samples and control using plate configuration tool. These systems are hybrids that allow you to choose between. 7°C (gradient heating design). 2. Figure 1. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. These robust readers have been placed in labs from. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Step 3. The reader measures both flat and round 96-well microplates and. Turn on Plate Reader and wait for it to complete its start-up routine. 0077 OD 0. , prior to compound addition) from the cells. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Our systems for high-content imaging (HCI) and high-content analysis (HCA) provide flexible scalability making it easy to evolve your system alongside your research. Incubate at RT. This can increase throughput and efficiency, thus saving time and space in the lab. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. Cellular Imaging Systems. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 306 plate air cal. 0) OD (0. Instrument settings can be saved as a protocol file. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. In . Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. Microplate Reader Shelf. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. #SpectraMax L Config) Methods. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535. . The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Learn more about how absorbance is measured, and some key applications that utilize absorbance. The luminescent reaction must be very intense for a reliable reading. 9000-0756. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 944. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. One plate carrier adapter is provided with the instrument. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. 1. Cet appareil ISM est confomre à la norme NMB-001 du Canada. The new assay will complement Molecular Devices’ workflow solution for cell line development by offering a 10-minute titer assay that can be run on the company’s SpectraMax series of microplate. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well. V. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Skip to main content 美谷分子仪器（上海）有限公司 咨询服务热线：400-821-3787. AXON Patch-Clamp. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Devices’ StakMax® Microplate Stacker in a matter of minutes and . (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. The CellTiter-Glo assay from Promega uses luciferase. • Analyze data in a single plate, across multiple plates, or between different groups. the standard plate reader applications, protecting your initial investment. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 2. TECHNICAL NOTE. Table 2 summarizes the results for the uniformity test. SpectraMax Readers have the most complete level of product validation and compliance. Your applications, your modes, your choice. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. View SoftMax Pro GxP. Figure 9. #S1205. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Published Dec 16, 2020 | Updated Dec 06, 2021. Turn on Plate Reader and wait for it to complete its start-up routine. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. com #THX1138 Introduction The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes. Multimode Microplate Readers. SoftMax® Pro 7. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofRead plate standard and mix. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. D. Supersedes 1. Published Dec 16, 2020 | Updated Nov 08, 2023. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software Check software compatibility with Windows 10 and locate your software installer. SoftMax Pro Software. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. A kinetic plate read was initiated at time zero. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Molecular Devices' microplate readers. (a) Chemical structures of 1 (HADP) and its analogues 2–4. An end-to-end solution for high-content imaging and analysis. M2. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 190. 315 can't find zero order. The former have been the traditional domain of plate readers and great strides have been. 5 mm hex key to loosen, but not remove, the hex screw that secures the filter to the filter slide. 1. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. F3/F5 Multi Mode Microplate Readers. This multi plate reader is excellent to work with for checking the U. Step 1: Turn on your microplate reader. Select the Driver tab. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. 340 PC384. Check pages 8-42 to 8-46 of the user manual. Keywords: spectramax ABS microplate readers, spectramax ABS plus microplate readers, spectramax ABS readers, ABS plus microplate readers, ABS. Perform the following steps to install the drivers: Start > Control Panel > System. coli stock with an OD 600 value of 0. Dual monochromators increase performance and assay flexibility. ensure your reader is thoroughly tested against specifications we consider critical to achieving quality results. Absorbance Plate Reader Comparison Chart. SoftMax® Pro 7. 0. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofWe would like to show you a description here but the site won’t allow us. Molecular Devices also offers Digital IQ/OQ/PM. White plates reflect the light, enabling even low levels of Luminescence to be detected. 50/50. Turn the reader on and secure the USB cable. ®ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. #4TI-0655) Microplates. SpectraMax® Microplate Readers and SoftMax® Pro Software. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Plate pre-read measurements were made in a Costar UV plate. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 3. 3T3 L1 adipocytes were plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours,Lower variance: Normalize your plate reader assay data to the number of cells in each well. #iD3) Methods. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. It is more inert and reduces compound interference. HTRF-compatible SpectraMax Multi-Mode Readers. Molecular Devices makes. 6 % of reading 4. #X7060. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Select Update Driver. Find our technical support information along with contact details. ABS2 Validation Plate Protocol Installation InstructionsEndpoint, dual read, kinetic, fast kinetic. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:[The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Category 2 and 3 microplate readers use gradient heating as well. The adapter is required for optimum performa nce with standard 96-well and 384-. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Step 4: Incubate at room temperature for 1 hour, protected from light. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 7°C (gradient heating design). The instrument supports the following read modes:. This can increase throughput and efficiency, thus saving time and space in the lab. Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. Category 2 and 3 microplate readers use gradient heating as well. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 5. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The primary mode is fluorescence. and fluorescence microplate readers—. Endpoint. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. These systems are hybrids that allow you to choose between. This offer includes SpectraMax i3x, SpectraMax. These models can no longer be repaired. The system provides automation for up to 50 microplates for easy walkaway automation. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. For a higher . SoftMax Pro. ELISA Plate Readers / Microplate Readers. Viewing results on your new SpectraMax iD3 Multi-Mode Microplate Reader. The Beacon Melamine Plate Kit is a competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of melamine in contaminated samples. FlexStation® 3 Multi-Mode Microplate Reader. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Instrument settings can be saved as a protocol file. Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. The SpectraTest® ABS1 Absorbance Validation Plate**4 °C above ambient to 45 °C. Fig 2: Enzymatic response of synthetic compounds. We would like to show you a description here but the site won’t allow us. SpectraMax Microplate Readers. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µl.